Structure-based calculation of excipient effects on the viscosity of concentrated antibody solutions

Computational prediction of the viscosity of therapeutic monoclonal antibodies (mAbs) at high concentration is highly desirable in the early discovery and development phases where the material needed for experimental determination is typically limited. Here, we present a unique coarse-grained (CG) simulation method that enables residue-level simulation of full-length antibodies with an elastic network, under simulated shearing force, to <i>de novo</i> predict viscosities of solutions of two distinct mAbs (an IgG1 and an IgG4), in the absence and presence of six excipients. Our results suggest the method can properly distinguish the viscosity profile of the two model mAbs, and directionally forecast viscosity change in response to added excipients. Furthermore, this CG modeling approach provides detailed protein-protein interaction mapping down to residue-level contacts, including contact lifetimes and nature of interactions, illuminating microscopic insights into the underlying molecular interactions. It serves as a valuable tool for viscosity prediction, mechanistic insights, and mitigation strategies.