Generation and characterization of a human induced pluripotent stem cell line (hiPSC) expressing the rEstus voltage sensor under doxycycline induction
The UKBi015-B-6 human induced pluripotent stem cell (hiPSC) line was generated by targeted insertion of a doxycycline-inducible TRE3G expression cassette encoding the ultrasensitive genetically encoded fluorescent voltage indicator rEstus into the AAVS1 locus. This hiPSC line enables controlled, reversible induction of rEstus expression to non-invasively monitor membrane potential dynamics in a human cellular background. The resulting platform allows high-throughput optical electrophysiology across diverse hiPSC-derived lineages, including cardiomyocytes, neurons, and non-excitable cells, thereby facilitating drug screening, developmental studies, and quantitative analysis of bioelectrical signaling under standardized, human-relevant conditions.
Authors
- Daniela Malan (ORCID: https://orcid.org/0000-0001-9034-6555)
- Philipp Rühl (ORCID: https://orcid.org/0000-0002-3554-3107)
- Bernd K. Fleischmann (ORCID: https://orcid.org/0000-0002-9202-8363)
- Caroline Geisen
- Stefan H. Heinemann (ORCID: https://orcid.org/0000-0002-4144-0251)
Institutions
- University of Bonn (DE)
- University Hospital Bonn (DE)
- Jena University Hospital (DE)
- Friedrich Schiller University Jena (DE)
Publication Details
- Journal
- Stem Cell Research
- Published
- 2026-03-21
- DOI
- https://doi.org/10.1016/j.scr.2026.103972
- Primary Topic
- Planarian Biology and Electrostimulation
- Type
- article
- Field-Weighted Citation Impact
- 0.00