Temperature-driven metabolic acceleration independent of cell growth enables high-level production of d-ribulose and shinorine in Streptomyces lividans

This study reveals that S. lividans exhibits enhanced glucose consumption and metabolite production at elevated temperatures, even under conditions of extensive cell death. We identified S. lividans as a novel producer of the rare sugar D-ribulose and successfully engineered it for high-level shinorine production under elevated temperature conditions. These findings highlight the potential of S. lividans as a promising host for the production of primary and secondary metabolites derived from the pentose phosphate pathway.