VISDB 2.0: A manually curated resource of viral integration sites and their regulatory maps in human diseases

The integration of viral sequence into the human genome plays critical role in the onset of various diseases, especially cancers. To facilitate systematic analysis of these events, we present VISDB 2.0, an expanded and updated database of human viral integration sites (VISs). The current release contains more than 270,000 manually curated VISs derived from 209 peer-reviewed publications, covering 11 viruses, including four retroviruses and seven DNA viruses. This represents a 3.5-fold increase in VIS entries compared with the previous version. Each VIS record includes standardized genomic coordinates and annotations describing local genomic context, CpG islands, repetitive elements, chromosomal fragile sites, non-coding RNA loci, regulatory elements, epigenomic features, and associated drug-target genes. All records are organized into structured datasets and are available for bulk download to support reuse and independent analysis. VISDB 2.0 also provides a web-based interface that enables querying, filtering, visualization, and retrieval of VISs across different viruses and genomic features. Together, these data constitute a comprehensive and traceable resource for investigating viral integration landscapes and their genomic associations.