A dual-duplex RT-dPCR surveillance strategy for discriminating avian and seasonal influenza viruses in wastewater
Abstract The highly pathogenic avian influenza (HPAI) A(H5Nx) viruses, specifically those of clade 2.3.4.4b, remain a major concern for global animal and public health. The current epidemiological situation in Europe and beyond reveal an alarming increase in spread and occurrence of these viruses, underscoring the need for enhanced surveillance and proactive intervention measures. We propose a dual duplex strategy (combining CDC Flu SC2 multiplex and JRC HA-JRC MP duplex assays) for discriminating avian and seasonal influenza viruses in wastewater, supporting rapid strain-specific environmental monitoring.
Authors
- Giuseppina La Rosa (ORCID: https://orcid.org/0000-0002-2657-100X)
- Marika Crimaudo (ORCID: https://orcid.org/0000-0001-6532-7969)
- Calogero Terregino (ORCID: https://orcid.org/0000-0002-7727-3336)
- Mauro Petrillo (ORCID: https://orcid.org/0000-0002-6782-4704)
- Antonio Marchini (ORCID: https://orcid.org/0000-0003-3157-4552)
- Valentina Panzarin (ORCID: https://orcid.org/0000-0002-9376-1612)
- Valentina Paracchini (ORCID: https://orcid.org/0000-0002-4847-9992)
- Sabrina Marciano
- Carolina Veneri (ORCID: https://orcid.org/0000-0002-4187-4471)
- Ursula Vincent (ORCID: https://orcid.org/0000-0002-8538-6488)
- Gerhard Buttinger (ORCID: https://orcid.org/0000-0003-2151-162X)
- Bernd Manfred Gawlik (ORCID: https://orcid.org/0000-0002-4406-5362)
- Elisabetta Suffredini (ORCID: https://orcid.org/0000-0001-7605-0539)
- Tobias Wiesenthal
- Clementina Elvezia Cocuzza
Institutions
- Istituto Superiore di Sanità (IT)
- European Commission (BE)
- Eidos (Italy) (IT)
- Joint Research Centre (IT)
- Istituto Zooprofilattico Sperimentale delle Venezie (IT)
- University of Milano-Bicocca (IT)
Publication Details
- Journal
- Zenodo (CERN European Organization for Nuclear Research)
- Published
- 2026-09-18
- DOI
- https://doi.org/10.5281/zenodo.19054949
- Primary Topic
- Influenza Virus Research Studies
- Type
- preprint