Study on the Synergistic Effect of Tannic Acid-Minocycline Hydrochloride Dual-Loaded Core-Shell Structured Nanofiber Membrane for Periodontal Tissue Repair

The biological invasion of periodontal pathogens has brought great difficulties to periodontal tissue repair. This study reported the feasibility of coaxial electrospinning to prepare tannic acid (TA) and minocycline hydrochloride (MH) double-loaded nanofiber membranes as periodontal tissue functional repair dressings. The TA-MH @ CSNF nanofiber membrane has the best mechanical properties and superhydrophilicity, which are 83% and 72.1% higher than the tensile strength and Young's modulus of the matrix, respectively. The improvement of mechanical properties is due to the enhancement of fiber cohesion and interfacial adhesion by TA and MH polar groups, and the synergistic optimization of fiber strength and toughness is realized. Wettability optimization is due to the synergistic effect of TA and MH hydrophilic groups. The TA-MH @ CSNF nanofiber membrane with dual loading of TA and MH has the best antibacterial and cell proliferation properties. Compared with the matrix, the antibacterial rate of TA-MH @ CSNF nanofiber membrane was close to 100%, and cell proliferation was increased by 18%. TA and MH destroy the integrity of the bacterial membrane structure and block the synergistic mechanism of bacterial proliferation, which is the main reason for improving the antibacterial property of the nanofiber membrane. Moreover, TA's mild cell proliferation and MH's efficient targeted antibacterial mechanism provide an ideal environment for cell proliferation.