Sustainability-based assessment using HPLC method for estimation of colchicine and dexamethasone for multi-disease treatment: application to laboratory-made combination and rat plasma

This is the first reported chromatographic method enabling simultaneous quantification of COL and DEX in pharmaceutical and plasma matrices together with a comprehensive, multi-tool sustainability assessment (including VIGI and EPPI). The method's short run time, high sensitivity, and favorable sustainability scores support its application in routine quality control and preclinical pharmacokinetic studies.