Polimorfizmi genov ACTN3, IL-6 in PPARA ter anaerobna zmogljivost pri odbojkarjih

The aim of this study is to determine the relationship between ACTN3 rs1815739, IL-6 rs1800795, and PPARA rs4253778 gene polymorphisms and anaerobic test parameters among volleyball players. A total of 22 volleyball players voluntarily participated in the study. DNA isolation from buccal epithelial cell samples were performed using a PureLink DNA isolation kit, following the manufacturer's instructions. The genotyping of ACTN3 rs1815739, IL-6 rs1800795, and PPARA rs4253778 gene polymorphisms were determined using the real-time polymerase chain reaction technique. Statistical analysis of the test results was performed using the SPSS 23 software package. Homogeneity of variance was assessed with the Levene test, and normal distribution was evaluated using the Shapiro-Wilk test. The One-Way ANOVA test was applied for the analysis of all parameters, and significance was set at p < 0.05. When the data were evaluated, no statistically significant differences were observed between PPARA rs4253778 and ACTN3 rs1815739 gene polymorphisms and anaerobic performance parameters (p>0.05). Statistically significant differences were observed between the IL-6 rs1800795 gene polymorphism and anaerobic performance parameters of back strength (p> 0.05). In conclusion, in volleyball, PPARA rs4253778 and ACTN3 rs1815739 gene polymorphisms did not cause differentiation in anaerobic performance parameters. However, IL-6 polymorphism caused differentiation only in the back strength parameter. Additionally, the good performance of athletes with different prevalent level of genotypes in the studied performance criteria does not fully reveal which performance outcomes are influenced by the gene or genotype distributions. It is understood that further studies are needed to better determine the gene-performance relationship.