Effect of ethanol extract of Lycoperdon lividum on burn injury induced in rat model

Thermal burn injury causes inflammatory cell infiltration in the dermis and epidermal thickening. Subsequent to a burn injury, numerous inflammatory mediators and cytokines, including interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α), transforming growth factor beta 1 (TGF-β1), and vascular endothelial growth factor (VEGF), are generated, subjecting all tissues to oxidative damage. The objective of the current study is to examine the therapeutic efficacy of ethanol extract of Lycoperdon lividum on burn injuries, by assessing the wound healing and the inflammatory response by the use of rat model that grouped into five groups each of fifteen rats that further sub-divided into three groups each of five rats that sacrificed at day five, ten and twenty, respectively. Area size of burn was measured at days five, ten and twenty of experiment. Skin biopsies was taken for tissue homogenate and histopathological studies. Levels of IL-10, IL-6 and TNF-α, TGF-β and VEGF were measured in the homogenate by enzyme linked immunosorbent assay (ELISA). Result showed that ethanol extract of Lycoperdon lividum has good anti- inflammatory, proliferative effect and shortening time of wound healing by decreasing proinflammatory mediator like IL-6 and TNF-α and enhance the expression of anti-inflammatory mediator like IL-10 also has modulatory effect on TGF- β and VEGF. Area size of burn measurement showed significant area contraction after treatment with ethanol extract ointment in comparison to other groups with a significant decrease in inflammatory marker after treatment with ethanol extract of Lycoperdon lividum. Histopathological finding showed that there is significant decrease in inflammation, granulation and significant increase in fibrous tissue after application of ethanol extract ointment in comparison to other groups. The findings of this study revealed that the ethanol extract of Lycoperdon Lividum exhibit significant burns healing activities.