Effect of yeast selenium on autophagy and PINK1/Parkin expression in Diquat-induced chicken liver and kidney cells

(Objective) This study aims to explore the effect of yeast selenium on alleviating autophagy and PINK1/Parkin expression in chicken liver and kidney cells induced by diquat. (Method) A total of 120 180-day-old Kangle Yellow chickens were randomly divided into 4 groups (5 replicates per group, 6 chickens per replicate): control group (A, basal diet + 15 mg/kg 0.9% normal saline), Diquat group (B, basal diet + 15 mg/kg diquat), Diquat + Se group (C, basal diet + 0.5 mg/kg yeast selenium + 15 mg/kg diquat), and selenium group (D, basal diet + 0.5 mg/kg yeast selenium + 15 mg/kg 0.9% normal saline). After a one-week adaptive feeding period, the experiment was conducted, and liver and kidney tissues were collected at the end. Immunohistochemistry, Western blotting, and real-time fluorescence quantitative PCR techniques were used to detect the mRNA and protein expression levels of LC3B, PINK1, and Parkin. (Result) The results showed that diquat significantly increased the mRNA and protein expression levels of LC3B, PINK1, and Parkin in chicken liver and kidney tissues (P < 0.05). The Diquat + Se group significantly reduced this trend, and there was no significant difference between the selenium group and the control group. Immunohistochemical results indicated that yeast selenium alleviated the overexpression and distribution of LC3B, PINK1, and Parkin in chicken liver and kidney induced by diquat. (Conclusion) Therefore, yeast selenium can alleviate diquat-induced autophagy in chicken liver and kidney cells, and the mechanism may be related to the PINK1/Parkin mitochondrial autophagy pathway.