Simultaneous thermal and large field-of-view two-photon imaging enables optimization of signal-to-noise
Concurrent thermal and LF-TPM imaging enables a quantitative optimization of laser power and SNR in LF-TPM systems. We demonstrated the feasibility of bilateral brain mapping using LF-TPM. These findings will help expand the utility of LF-TPMs for mesoscopic brain mapping applications.
Authors
- Jonathan Bumstead (ORCID: https://orcid.org/0000-0003-1254-7938)
- Annie R. Bice
- Ruben A. Garcia-Reyes (ORCID: https://orcid.org/0000-0002-0056-926X)
- Daniel C. Castro (ORCID: https://orcid.org/0000-0001-8036-1313)
- Shengxuan Chen (ORCID: https://orcid.org/0000-0001-9093-6379)
- Hunter Banks
- Seana H. Bice
- Joseph P. Culver
Institutions
- Washington University in St. Louis (US)
Publication Details
- Journal
- Neurophotonics
- Published
- 2026-03-11
- DOI
- https://doi.org/10.1117/1.nph.13.1.015014
- Primary Topic
- Advanced Fluorescence Microscopy Techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.00