Morphological and molecular characterization of Sphingomonas lindongxini sp. nov. and Sphingosinicella wutangchuni sp. nov., isolated from soil in the Qinghai-Tibetan Plateau
Four Gram-stain-negative, non-spore-forming, motile, short rod-shaped strains (LY29<sup>T</sup>, LY160, LY54<sup>T</sup> and LY1275) were isolated from soil of the Qinghai-Tibetan Plateau, China. Based on 16S rRNA gene sequences, strain pairs LY29<sup>T</sup>/LY160 and LY54<sup>T</sup>/LY1275 were identified as members of the genera <i>Sphingomonas</i> and <i>Sphingosinicella</i>, respectively, with highest similarities to <i>Sphingomonas alba</i> SE158<sup>T</sup> (97.8%) and <i>Sphingomonas montis</i> DRJ-4<sup>T</sup> (98.0%), respectively. In phylogenomic analyses based on 426 core gene sequences, LY29<sup>T</sup> and LY160 clustered with <i>Sphingomonas rhizophila</i> THG-T61<sup>T</sup>, while LY54<sup>T</sup> and LY1275 were closely related to <i>Sphingosinicella humi</i> QZX222<sup>T</sup>, <i>Sphingosinicella flava</i> UDD2<sup>T</sup> and <i>Sphingosinicella vermicomposti</i> KCTC 22446<sup>T</sup>. Digital DNA-DNA hybridization and average nucleotide identity values between the four strains and their closest relatives were determined to be below 24.2% and 80.49%, respectively, thus falling below the recommended species delineation thresholds (70% and 95-96%, respectively). The genomic DNA G+C contents of strains LY29<sup>T</sup> and LY54<sup>T</sup> were 64.8 and 66.8 mol%, respectively. Strain LY29<sup>T</sup> contained predominant fatty acids C<sub>16</sub> (C<sub>16:1</sub> <i> ω</i>7c and C<sub>16:1</sub> <i> ω</i>6c) and C<sub>18</sub> (C<sub>18:1</sub> <i> ω</i>7c and C<sub>18:1</sub> <i> ω</i>6c), while LY54<sup>T</sup> had iso-C<sub>16:0</sub> as the major fatty acid. Both strains shared major polar lipids (diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified sphingoglycolipid). Strain LY29<sup>T</sup> possessed ubiquinone Q-10 and homospermidine, whereas LY54<sup>T</sup> contained MK-9(H<sub>2</sub>) as the major quinone. Based on a polyphasic taxonomic approach, type strains LY29<sup>T</sup> (=GDMCC 1.4177<sup>T</sup>=JCM 36472<sup>T</sup>) and LY54<sup>T</sup> (=GDMCC 1.4236<sup>T</sup>=KCTC 8630<sup>T</sup>) represent novel species of the genera <i>Sphingomonas</i> and <i>Sphingosinicella</i>, for which the names <i>Sphingomonas lindongxini</i> sp. nov. and <i>Sphingosinicella wutangchuni</i> sp. nov. are proposed.
Authors
- Feifei Wang (ORCID: https://orcid.org/0009-0009-4448-9243)
- Xiaorui Liu (ORCID: https://orcid.org/0009-0001-8258-5457)
- Qiang Hou (ORCID: https://orcid.org/0000-0002-7874-454X)
- Huimin Zhou (ORCID: https://orcid.org/0000-0003-3481-9025)
- Shuo Ning
- Jiajia Ma
- Ji Pu
- Caixin Yang
- Chunmei Liu
- Shan Lu
- Jing Yang
- Linglin Cao
- Hao Zhou
- Yuyuan Huang
- Jianguo Xu
Institutions
- Guangxi Medical University (CN)
- Shanxi Medical University (CN)
- National Institute for Communicable Disease Control and Prevention (CN)
- Hebei Provincial Center for Disease Control and Prevention (CN)
Publication Details
- Journal
- INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
- Published
- 2026-03-11
- DOI
- https://doi.org/10.1099/ijsem.0.007094
- Primary Topic
- Genomics and Phylogenetic Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00