Whole-genome sequencing of <i>Escherichia coli</i> DH5α using MinION nanopore technology
We present the whole-genome sequence of <i>Escherichia coli</i> DH5α, a laboratory strain integral to molecular biology applications. We assembled a high-quality genome using Qiagen's DNeasy Blood and Tissue Kit and the Oxford Nanopore MinION platform (~4.58 Mb, 50.8% guanine-cytosine [GC] content), offering insights into genetic structure and functional characteristics.
Authors
- T.G. Satheesh Babu
- M Karthic Krishna
- Megha S. Kumar (ORCID: https://orcid.org/0000-0003-4391-5246)
- P. V. Suneesh
- K.P. Soman
Institutions
- Amrita Vishwa Vidyapeetham (IN)
Publication Details
- Journal
- Microbiology Resource Announcements
- Published
- 2026-03-12
- DOI
- https://doi.org/10.1128/mra.01076-25
- Primary Topic
- Nanopore and Nanochannel Transport Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00