UAV Multispectral Imagery Combined with Canopy Vertical Layering Information for Leaf Nitrogen Content Inversion in Cotton

Leaf nitrogen concentration (LNC) exhibits pronounced vertical heterogeneity across canopy layers, which affects the accuracy of nitrogen diagnosis derived from UAV-based remote sensing imagery. To address the differential contributions of leaf nitrogen from distinct canopy strata and the limitations associated with single-source features, this study proposes an integrated framework that combines cumulative LNC indicators across canopy layers with multi-source feature sets (vegetation indices and texture features). Centered on three core technical innovations—(1) incorporating canopy-layer aggregation logic into LNC modeling, (2) integrating spectral and structural information through CNN-based feature fusion, and (3) combining deep feature extraction with gradient boosting regression to improve robustness under multi-stage conditions—the framework systematically evaluates three machine learning algorithms: Random Forest (RF), a Convolutional Neural Network–Extreme Gradient Boosting hybrid model (CNN_XGBoost), and K-Nearest Neighbor (KNN) for cotton LNC estimation across multiple growth stages. The results demonstrate that cumulative canopy-layer nitrogen indicators more effectively represent overall plant nitrogen status than single-layer measurements. The integration of multi-source features further enhances model performance. Under both single-variable inputs and combined VI–TF feature sets, the CNN_XGBoost model consistently outperforms the other models in calibration accuracy and stability across all growth stages. Its optimal performance occurs during the cotton flowering and boll stage, achieving a calibration R2 of 0.921. Overall, the proposed framework substantially improves the estimation accuracy of cotton LNC and provides both a theoretical foundation and technical support for precision nitrogen management and sustainable agricultural development.