The interplay between temperature and growth phase shapes the transcriptional landscape of <i>Pseudomonas aeruginosa</i>

<i>Pseudomonas aeruginosa</i> is a highly adaptable opportunistic pathogen with a repertoire of mechanisms for surviving in diverse and often challenging environments, yet it is most studied at 37°C as the optimum temperature for growth. To better understand how this bacterium survives in the environment versus the human body, we performed transcriptomics on <i>P. aeruginosa</i> grown at 25°C and 37°C. At each temperature, we examined both exponential and stationary phases and determined the LasRI quorum sensing regulon at 37°C compared to 25°C using a Δ<i>lasR</i> mutant, which uncovered a suite of previously unrecognized LasR-regulated genes. Our work provides a comprehensive transcriptomic resource for the thermoregulation of <i>P. aeruginosa</i> at two growth phases, as well as growth phase and LasR regulation at two temperatures.