Optimizing ovine brucellosis serodiagnosis: evaluation of recombinant Brucella antigens and multi-antigen combinations for iELISA

The three-antigen combination (rOmp19 + rOmp31 + rBP26) identified in this study achieves an optimal balance between sensitivity and specificity, supporting its potential to enhance the performance of brucellosis serodiagnosis. This work provides experimental evidence and constitutes an important step toward the development of multi-antigen iELISA-based brucellosis detection kits. However, further validation and optimization are required before clinical or field application.