Ribosome Molecular Aging Shapes Translation Dynamics
A pulse-chase labeling strategy enables mapping subcellular demographics of macromolecular complexes in space and time.Molecular aging of ribosomes drives differential mRNA translation and shapes elongation dynamics.The collision propensity of specific ribosome subpopulations increases with molecular age.Older ribosomes shape translation dynamics during organismal aging.
Authors
- Lifei Jiang (ORCID: https://orcid.org/0000-0001-5390-3068)
- Claire J. Weaver (ORCID: https://orcid.org/0000-0001-6386-5341)
- Qiwei Yu
- Maya S. Butani
- Lennard W Wiesner (ORCID: https://orcid.org/0000-0001-7161-4358)
- Andrej Košmrlj (ORCID: https://orcid.org/0000-0001-6137-9200)
- Troy J. Comi (ORCID: https://orcid.org/0000-0002-3215-4026)
- Margaret S. Ebert (ORCID: https://orcid.org/0000-0002-9122-7854)
- Aya A. Abu-Alfa
- Peter J. Metzger (ORCID: https://orcid.org/0000-0001-5172-9174)
- Jordy F. Botello (ORCID: https://orcid.org/0000-0002-0163-0595)
- Minkyu Lee
Institutions
- Howard Hughes Medical Institute (US)
- Princeton University (US)
Publication Details
- Journal
- bioRxiv (Cold Spring Harbor Laboratory)
- Published
- 2026-03-09
- DOI
- https://doi.org/10.64898/2026.03.08.710403
- Primary Topic
- RNA and protein synthesis mechanisms
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Science Foundation
- Howard Hughes Medical Institute
- Princeton University
- Princeton Center for Complex Materials
- National Institutes of Health
- National Institute of Allergy and Infectious Diseases
- Division of Materials Research