SIMULTANEOUS DETECTION OF CYTOMEGALOVIRUS AND MYCOPLASMA NUCLEIC ACIDS IN ALVEOLAR LAVAGE FLUID OF CHILDREN WITH RESPIRATORY TRACT INFECTION

Objective: To investigate the infection of cytomegalovirus and Mycoplasma pneumoniae in alveolar lavage fluid of children with respiratory tract infection. Methods: A total of 31 children with respiratory tract infection were enrolled in the Department of Pediatrics, the First Affiliated Hospital of Anhui Medical University from May to August in 2017, and the cytomegalovirus nucleic acid in the alveolar lavage fluid was detected by real-time fluorescent polymerase chain reaction. Ribonucleic Acid (RNA) thermostatic amplification technology was used to detect Mycoplasma pneumoniae nucleic acid in the alveolar lavage fluid. Results: The total detection rate of both pathogens was 64.52%, the positive rate of cytomegalovirus nucleic acid was 38.71%, and the positive rate of Mycoplasma pneumoniae was 25.81%. Conclusion: Cytomegalovirus and Mycoplasma pneumoniae have high infection rate in children with respiratory tract infection. The combined detection of these two infection agents in alveolar lavage fluid has important application value for clinical etiology and treatment