Autofluorescence lifetime imaging resolves cell heterogeneity within peripheral blood mononuclear cells
We demonstrate that autofluorescence lifetime imaging can resolve functional and phenotypic metabolic subpopulations within a mixed culture of immune cells from human blood. This provides a new technique to characterize metabolic activity within immune cells from the peripheral blood of patients, which could improve disease diagnostics and the production of cell therapies.
Authors
- Jeremiah Riendeau (ORCID: https://orcid.org/0000-0002-3014-2849)
- Elizabeth M. Maly
- Kayvan Samimi (ORCID: https://orcid.org/0000-0002-2566-612X)
- Melissa C. Skala (ORCID: https://orcid.org/0000-0002-6320-7637)
- Lucia Hockerman
Institutions
- University of Wisconsin–Madison (US)
- Morgridge Institute for Research (US)
Publication Details
- Journal
- bioRxiv (Cold Spring Harbor Laboratory)
- Published
- 2026-03-08
- DOI
- https://doi.org/10.64898/2026.03.06.710224
- Primary Topic
- Advanced Fluorescence Microscopy Techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.00