The complete mitochondrial genome sequence of the angel-wing mushroom <i>Pleurocybella porrigens</i>

The mushroom <i>Pleurocybella porrigens</i> is widely distributed in temperate regions of the Northern Hemisphere. However, in the autumn of 2004, 59 food-poisoning incidents were reported in Japan, resulting in 17 deaths due to acute encephalopathy. In previous studies, we demonstrated that a lectin (PPL), pleurocybelline (PC), and pleurocybellaziridine (PA) from this mushroom caused neurotoxic effects in mice, and we also reported its nuclear genome (2013, 2026) and transcriptome data from fruiting bodies and mycelia (2024). Here we report the complete mitochondrial genome sequence of <i>P. porrigens</i>. The mitochondrial genome of <i>P. porrigens</i> is 63,054 bp in length and contains 18 protein-coding genes, 2 rRNA genes, and 25 tRNA genes. Phylogenetic analysis based on 14 conserved mitochondrial protein-coding genes recovered <i>P. porrigens</i> as a distinct and well-supported lineage within Agaricales. In the inferred topology, <i>P. porrigens</i> was positioned basal to the core Agaricales clade, internal to <i>Clavaria fumosa</i> and <i>Hygrophorus russula</i>, and clearly separated from Boletales outgroup taxa. This phylogenetic placement is consistent with recent phylogenomic frameworks recognizing Phyllotopsidaceae as an independent lineage within Agaricales. This study represents the first report of a complete mitochondrial genome from the genus <i>Pleurocybella</i> and provides a valuable reference for future comparative mitochondrial genomic studies within Agaricales.