Reprogramming of neuronal genome function and phenotype by astrocytes

Neuronal gene expression and chromatin accessibility landscape are profoundly remodeled by astrocytes over weeks of co-cultureAstrocyte-responsive neuronal gene modules and neuron-responsive astrocytic gene modules are enriched for genes associated with schizophrenia and familial Alzheimer's DiseaseSingle-cell CRISPR interference and activation screens of astrocyte-responsive gene regulatory elements identified dozens of functional regulatory elements of TF genes in neuronsSingle-cell CRISPR interference and activation screens of >200 astrocyte-responsive TF genes uncovered discrete functional clusters that promote neuronal maturity or stemnessAstrocyte-responsive TF genes reprogram neuronal electrophysiology and neurite morphology.