Electro‐Mechanically Agitated Nanocomposite Films for High‐Performance Enzymatic Bioanodes in Sustainable Biofuel Cells

ABSTRACT The present work on a novel electro‐mechanical agitation‐based nanocomposite bioanode has been developed to be used in the performance‐based enzymatic biofuel cells (EBFCs). This nanocomposite incorporates zinc‐blende nanodots (ZBNDs), silver nanostrands (AgNS), multiwalled carbon nanotubes (MCNTs), as well as polyaniline (PANi), which is prepared by a green route using a neem ( Azadirachta indica ) leaf extract as the reducing and stabilizing agent. During synthesis, uniform dispersion and controlled formation of nanostructures and increased interfacial synergy among components were made possible by electro‐mechanical agitation, which increased enzyme immobilization and electron transfer. The study methodically assesses the structural features, catalytic performance, stability of operations, and usability of the synthesized nanocomposite. The material was thoroughly characterized by material characterization (FTIR, XRD, EDX, SEM, and TEM) that indicated the creation of a porous, conductive structure, with the electrochemical analysis (CV, EIS, LSV) revealing a maximum current density of 8.56 mA/cm 2 and a power output of 320 mW/cm 2 which was higher than the majority of conventional EBFC bioanodes. There was also great reproducibility and long‐term stability in the use of the bioanode. It is a new synthesis pathway, a synthesis involving agitation‐assisted dispersion of a rarely studied strategy in conductive polymer nanocomposites using green chemistry. The developed bioanode has two potential applications in sustainable energy and biomedical technologies, and in addition to energy harvesting, it can be used in self‐powered biosensing devices.