Statistical Optimization of Hydrothermal Conversion of Stevia rebaudiana Residues for Sustainable Production of 5-HMF and Furfural as Platform Chemicals

In recent years, the sustainable production of bio-based platform chemicals from non-lignocellulosic biomass has garnered increasing attention. In this study, <i>Stevia rebaudiana</i> residues were evaluated via hydrothermal liquefaction (HTL) to produce key furan derivatives, namely 5-hydroxymethylfurfural (5-HMF) and furfural. The effects of reaction temperature (160-240 °C) and pressure (0-8 MPa) on product yields were systematically investigated and statistically evaluated using Analysis of Variance (ANOVA) and regression modeling. The highest 5-HMF (93.1 mg/L) and furfural (51.2 mg/L) yields were obtained at 200 °C, while pressure was found to have no statistically significant effect on product formation. To elucidate the physicochemical transformations occurring during hydrothermal processing, Scanning Electron Microscopy (SEM) and Fourier Transform Infrared (FT-IR) spectroscopy were used to analyze the morphological and functional group evolution of the biochar and bio-oil fractions. SEM images revealed gradual structural degradation, pore formation, and carbonization with increasing temperature, while FT-IR analysis confirmed dehydration, hydrolysis of glycosidic bonds, aromatization, and the formation of carbonyl groups directly related to furan production. A validated High-Performance Liquid Chromatography (HPLC-UV) method providing analytical efficiency for the precise determination of 5-HMF and furfural in complex biomass matrices was developed. This study provides a comprehensive understanding of the thermochemical behavior of <i>Stevia rebaudiana</i> biomass by integrating morphological characterization, molecular-level spectroscopy, and statistical process modeling and establishes a predictive framework for optimizing furan production under hydrothermal conditions. The findings highlight the potential of <i>Stevia rebaudiana</i> residues as a sustainable feedstock within circular bioeconomy strategies and offer a scalable approach for converting agricultural waste into high-value platform chemicals.