Hybrid Whole-Genome Sequencing for Genetic Stability Assessment of Infectious Laryngotracheitis Virus Vaccine Strains

Collectively, these findings show that a hybrid WGS workflow can generate high-confidence genome assemblies for the specific commercial ILTV vaccine vials analyzed and can support QC-relevant detection of major structural variations. Because this study is cross-sectional (two strains; single lot/vial per strain), it cannot distinguish potential biological lot-to-lot variation from methodological differences, and a comprehensive genetic stability evaluation will require applying this workflow across defined passage levels and/or multiple production lots.