A New Kinetic‐Mechanistic Approach Towards Spectrophotometric Bioanalysis of 2‐Phenylethylamine as a Sympathomimetic Agent Using Sodium Isopropyl Xanthate

2-Phenylethylamine (2-PEA), an aromatic biogenic amine, demonstrates significant physiological and toxicological effects on human health. A variety of conventional methods have been widely used for the determination of 2-PEA. However, the necessity of complex sample preparation and high cost of instrumentation may pose challenges to their widespread application. The application of sodium isopropyl xanthate (SIPX) in spectrophotometric method was introduced a novel optical probe for simple, fast and cost-effective detection of 2-PEA. The distinct UV-Vis spectrum of SIPX is changed in the presence of the target molecule (2-PEA) which confirmed successful application of sensing strategy. Under optimal conditions, a linear range were achieved between 0.4 and 50 mM. The developed probe was characterized by dynamic light scattering (DLS) and zeta potential tests. The selectivity of prepared probe was validated by its minimal effects towards aliphatic amines, including histamine, cadaverine, 1,4-diaminobutane, spermidine and ethylenediamine. Furthermore, the validation of reported analytical approach was examined in real human plasma. It can be deduced that SIPX as a novel optical probe could offer suitable, reliable, affordable and rapid sensing platform for detection of 2-PEA in real samples. This study introduced significant innovations, including the development of a new organic optical probe for biogenic amine recognition in real samples, the establishment of a simple and rapid analytical workflow and the presentation of a novel spectrophotometric sensing platform offering selective detection of 2-PEA. These advancements highlight clear technical benefits of this opto-sensors for the specific recognition of biogenic amines compared with conventional analytical methods.