Diagnostic Accuracy of a CRISPR-Based Assay in Smear- and Culture-Negative Fungal Keratitis
In this diagnostic study, in patients with smear- and culture-negative FK, the RID-MyC assay showed good diagnostic accuracy comparable with IVCM and was feasible in all cases, including those in whom imaging was not possible. With its rapid turnaround and minimal equipment needs, RID-MyC may serve as a practical adjunct to conventional diagnostics, particularly in high-burden, resource-limited settings where IVCM is unavailable or contraindicated.
Authors
- Siddharth Narendran (ORCID: https://orcid.org/0000-0002-9220-8049)
- Tos T. J. M. Berendschot (ORCID: https://orcid.org/0000-0002-8101-939X)
- Jaishree Pandian
- Sankha Amarakoon (ORCID: https://orcid.org/0000-0002-9467-7519)
- Ram Rammohan
- Hanith Raj Deivarajan
- Dharsini Nandhakumar
- Divya Nachammai
- Deepa Jaisankar
- Jayashree Sivagnanam
- Balaji Ganapathi
- Venkatesh N. Prajna
- Lalitha Prajna
- Anita Raghavan
- Sujay Sanjay Jaju
Institutions
- Aravind Eye Hospital (IN)
- Maastricht University (NL)
Publication Details
- Journal
- JAMA Ophthalmology
- Published
- 2026-03-05
- DOI
- https://doi.org/10.1001/jamaophthalmol.2026.0113
- Primary Topic
- Ocular Infections and Treatments
- Type
- article
- Field-Weighted Citation Impact
- 0.00