ANTI CANDIDAL ACTIVITY OF GREEN TEA (CAMELLIA SINENSIS) EXTRACT AGAINST MDR CANDIDA SP. ISOLATED FROM SOLID WASTE OF LOCAL MARKET

The increase of the irrational use of antifungal drug has been a major cause of the development of multidrug-resistant (MDR) Candida species, which represents a threat to the limited activity of conventional antifungals. The research article examines the possibility of using green tea extract as a natural phytochemical counter-measure of MDR Candida in planktonic and sessile form. In the present research, the selective agar was used to isolate Candida sp from solid waste disposal of local market and analyze its MDR through resistance profiling. The crude extract of Camellia sinensis (used as green tea) was applied in this experiment and are characterized using Thin Layer Chromatography (TLC). To check the efficiency, different drug screening assays were conducted, such as cup plate assay, MTT assay, MIC assay and the anti-biofilm assay. We have found that green tea bioactive compounds and more so catechins have an important role in disrupting the biofilm structure of fungi. Overall, this study recommends the application of the green tea extract as an effective, natural, and readily available intervention on the management of MDR Candida infection. We provide a new and efficient alternative, which can be used in clinical practice as a complementary or an alternative of the current antifungal treatments using the inherent antifungal properties of green tea.