Re-examining hidden fitness: Female preferences for long-path songs in zebra finches

Female songbirds can evaluate male quality from secondary sexual characteristics such as songs. Studies have shown mixed findings on which acoustic features contribute to song attractiveness. In a 2024 study, Alam and colleagues introduced a holistic measure of song called "path length" and found that female zebra finches (Taeniopygia guttata) prefer long path length songs as compared to short path length songs. The original female-playback study was criticized for design limitations, notably its small sample size, and pseudoreplication. Here, we conducted a preregistered replication and extension study of the female preference for long vs. short path length songs. Our study will evaluate the robustness and generality of this preference.