Comparative evaluation of RNA isothermal amplification-gold probe lateral flow assay and targeted next-generation sequencing for the detection of Mycoplasma pneumoniae and influenza A and B viruses in children

tNGS offers high-throughput, high-sensitivity screening, while RGT is rapid, user-friendly, cost-effective and superior for identifying active infections and monitoring treatment responses. Optimal method selection is dependent on clinical scenarios and diagnostic priorities.