Antibody elution methods for multiplex immunofluorescence of Alzheimer's disease pathology in human post-mortem brain tissue
Employing β-mercaptoethanol (BME)-containing stripping buffer instead of heat-induced epitope retrieval gave similar results for both techniques in both short-term and long-term fixed tissue. However, iterative imaging sessions between cycles for traditional mIF still pose a greater risk for malalignment of target molecules in composite images.
Authors
- Julia Stevens (ORCID: https://orcid.org/0000-0001-9613-6188)
- M.G. Novelli
- Dhiraj Maskey
- Hoang-Tuong Nguyen-Hao
- Caine C. Smith
- Greg T. Sutherland
Institutions
- The University of Sydney (AU)
- UNSW Sydney (AU)
Publication Details
- Journal
- Frontiers in Neurology
- Published
- 2026-03-04
- DOI
- https://doi.org/10.3389/fneur.2026.1760600
- Primary Topic
- Advanced Fluorescence Microscopy Techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- University of Sydney
- National Institutes of Health
- National Institute on Alcohol Abuse and Alcoholism