The Protective Effect of Coenzyme Q10 on Serum Anti-Müllerian Hormone Levels in Rats Treated with Cyclophosphamide

Anti-Müllerian hormone (AMH) is widely used in veterinary medicine to assess reproductive health. Cyclophosphamide (CP) is a chemotherapeutic and immunosuppressive drug that has been reported to reduce AMH levels and cause damage to ovarian reserves, although findings vary depending on study design. Coenzyme Q10 (CoQ10), known for its antioxidant properties, has been suggested to have a protective role against oxidative stress-related ovarian damage. This study was conducted to examine how CP affects serum AMH levels in rats and whether CoQ10 supplementation could help reduce any negative impact. A total of 35 Wistar Albino rats were used in the study. They were divided into five groups: control, sham, CP, CoQ10, and CP+CoQ10. All substances were administered by oral gavage. CP was dissolved in distilled water and administered at a dose of 6 mg/kg/day, while CoQ10 was dissolved in olive oil and given at a dose of 2.8 mg/kg/day. The treatments lasted for four weeks. Results showed that CP alone did not significantly change AMH levels (p>0.05). In contrast, CoQ10 alone led to a significant increase in serum AMH levels (p<0.05). However, when CP and CoQ10 were given together, no significant difference in AMH levels was observed. This may suggest that CP interferes with or masks the effect of CoQ10 on the ovaries. These findings may contribute to a better understanding of the interaction between CP and CoQ10, especially in terms of dosage, duration and timing of treatment, in future studies.