Cricket Oil-Based Sunscreen Systems: Formulation Design, Ultraviolet Protection Performance, and Preclinical Safety Evaluation

<b>Background/Objectives:</b> Insect oils have gained attention as sustainable cosmetic ingredients due to their bioactive lipid content. This study aimed to characterize oils from cricket and to evaluate their safety, biological activities, and performance in sunscreen formulations. <b>Methods:</b> Oils were extracted from <i>Gryllus bimaculatus</i>, <i>Teleogryllus mitratus</i>, and <i>Acheta domesticus</i> by cold pressing following hot-air drying. Fatty acid composition was determined using gas chromatography-mass spectrometry. Safety was assessed by cytotoxicity testing in normal human dermal fibroblasts (NHDF) and the hen's egg chorioallantoic membrane (HET-CAM) assay. Antioxidant and anti-inflammatory activities were evaluated by intracellular reactive oxygen species (ROS) and nitric oxide (NO^•) assays. Based on biological performance, <i>T. mitratus</i> oil (TMO) was incorporated into sunscreen creams containing physical and chemical ultraviolet (UV) filters. Physical stability, viscosity, pH, sun protection factor (SPF), persistent pigment darkening/ultraviolet A protection factor (PPD/UVA-PF), and blue light protection were evaluated. <b>Results:</b> All cricket oils were non-cytotoxic to NHDF cells and were classified as non-irritating in the HET-CAM assay. TMO exhibited the strongest antioxidant activity, reducing intracellular ROS and significantly inhibiting NO^• production in lipopolysaccharide-stimulated cells. Only TMO showed measurable UVA protection (PPD/UVA-PF = 12.1, PA+++). Sunscreen creams formulated with TMO achieved higher photoprotective efficacy than olive oil-based creams, with SPF values up to 40.51 and PPD/UVA-PF up to 39.17. The inclusion of foundation pigments further increased SPF to 43.09 and enhanced blue light protection to 35.1%. <b>Conclusions:</b> TMO is a safe and effective multifunctional ingredient that enhances sunscreen performance and supports sustainable cosmetic formulation.