Lon protease-mediated secretome profiling in <i>Klebsiella pneumoniae</i>

Lon protease is a global regulator in bacteria affecting biofilm formation, antibiotic resistance, toxin-antitoxin complexes, and proteolysis of damaged proteins. In <i>Klebsiella pneumoniae</i>, Lon has roles in iron response, protein transport, and membrane assembly. Here, we apply bottom-up proteomics to identify proteins in the extracellular environment of WT and Δ<i>lon K. pneumoniae</i>.