Expedient MPLC-based isolation of lupane triterpenoids from <i>Glochidion zeylanicum</i> var. <i>tomentosum</i> (Dalzell) Trimen. Bark

An automated medium-pressure liquid chromatography (MPLC) system was employed for the preparative-scale isolation of lupane-type triterpenoids from <i>Glochidion zeylanicum</i> var. <i>tomentosum</i> (Dalzell) Trimen. Seven compounds were isolated and identified as glochidone (1), glochidonol (2), glochidiol (3), lupeol (4), epilupeol (5), lup-20(29)-ene-3α,23-diol (6) and lup-20(29)-ene-1α,3β-diol (7). The antimicrobial activity of these compounds was evaluated against <i>Micrococcus luteus</i>, <i>Staphylococcus aureus</i>, <i>Aspergillus fumigatus</i> and <i>Rhizopus oryzae</i> using the minimum inhibitory concentration (MIC) method. Glochidiol (3) showed moderate activity against <i>S. aureus</i> (64 µg/mL) and <i>R. oryzae</i> (32 µg/mL). Compound 6 also showed moderate antifungal activity against <i>A. fumigatus</i> (64 µg/mL). The results highlight the efficiency of MPLC and the influence of hydroxyl substitution on antimicrobial activity.