A dietary blend of prebiotic, probiotic, and organic acids has been demonstrated to be beneficial for Nile tilapia juveNiles

This study evaluated the impact of incorporating a blend of prebiotic, probiotic, and organic acids on the diet of Nile tilapia juveNiles and its influence on the performance of zootechnical, bromatological, and blood biochemical parameters, digestive and liver enzymes, and histological analysis of the intestine, liver, and muscle. A total of 150 animals ( $$44.89 \pm 0.82$$ g and $$12.88 \pm 0.12$$ cm) were distributed in a randomized design across 15 tanks (200 L) with water recirculation, with three replicates per treatment and over 30 days. The treatments consisted of a commercial 35% protein feed enriched with a blend of additives consisting of prebiotic, probiotic, and organic acids. The control group was designated as T1, and the experimental groups as T2 (2.50 g kg−1), T3 (5.00 g kg−1), T4 (7.50 g kg−1), and T5 (10.0 g kg−1). The incorporation of additives did not impact on performance parameters, but differences between groups were observed in the enzymes lipase, alkaline phosphatase (AP), superoxide dismutase (SOD), and alanine aminotransferase (p < 0.05). The blood biochemical analysis showed significant differences between groups in cholesterol, triglycerides, and creatinine. Animals in the T5 group showed the best results in terms of lipase, AP, catalase, and SOD enzyme activity. In conclusion, dietary supplementation with a blend of prebiotic, probiotic, and organic acids modulated specific physiological parameters in Nile tilapia juveNiles, particularly biochemical markers and enzyme activities, while growth performance and tissue histology remained unaffected. These findings support further research on functional feed additives in aquaculture.