Target‐site and non‐target‐site mechanisms confer multiple herbicide resistance in waterhemp ( <scp> <i>Amaranthus tuberculatus</i> </scp> ) accessions from Wisconsin
Metabolic resistance to 2,4-D and atrazine suggests that the use of other herbicides may have contributed to the selection of enhanced P450s and GSTs activity in A101 accession. To our knowledge, this is the first report of P450s associated with atrazine resistance in A. tuberculatus globally. A101 is the first confirmed case of A. tuberculatus resistance to hydroxyphenyl pyruvate dioxygenase inhibitors in Wisconsin, exhibiting a low-level resistance likely associated with P450s and GSTs activity. Our results suggest the coexistence of target-site resistance and NTSR mechanisms associated with glyphosate resistance in A101. © 2026 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Authors
- David E. Stoltenberg (ORCID: https://orcid.org/0000-0002-5265-3746)
- Isabel Schlegel Werle (ORCID: https://orcid.org/0000-0002-7133-3772)
- Damilola A. Raiyemo (ORCID: https://orcid.org/0000-0002-6521-2333)
- Rodrigo Werle (ORCID: https://orcid.org/0000-0003-1132-461X)
- Jed B. Colquhoun (ORCID: https://orcid.org/0000-0002-8160-293X)
- P. A. Tranel
- Alexander J. Lopez
- Mark J. Renz
- Felipe A Faleco
Institutions
- University of Wisconsin–Madison (US)
- University of Illinois Urbana-Champaign (US)
Publication Details
- Journal
- Pest Management Science
- Published
- 2026-03-02
- DOI
- https://doi.org/10.1002/ps.70672
- Primary Topic
- Weed Control and Herbicide Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00