Patient-derived complex organoids are a critical tool to understand and explore pediatric malignant brain tumors
Pediatric malignant brain tumors (PMBTs) remain among the most common and challenging cancers in children and adolescents, with current therapies often failing to deliver satisfactory outcomes. A major obstacle is their intrinsic and extrinsic resistance mechanisms, underscoring the urgent need for innovative therapeutic strategies and accurate preclinical modeling. Recent advances in organoid-based technologies offer promising tools to mimic PMBTs more faithfully in vitro. These three-dimensional (3D) models can replicate key features of the tumor and its brain-like microenvironment, providing valuable platforms for studying resistant cancer cells and testing novel treatment approaches. This review discusses the relevance of cultured 3D systems, organoids and tumoroids, in pediatric neuro-oncology, emphasizing their role in precision medicine. These models have become essential for dissecting the complex biology and dynamic biological processes of all PMBTs, while bridging clinical challenges with experimental discoveries, ultimately enabling more effective and personalized treatments for young patients.
Authors
- Antony Joud
- Sophie Martin (ORCID: https://orcid.org/0000-0002-6156-979X)
- Érika Cosset (ORCID: https://orcid.org/0000-0001-5481-6556)
- Marie Duhamel (ORCID: https://orcid.org/0000-0002-4006-5605)
- Marlène Deschuyter (ORCID: https://orcid.org/0009-0003-5304-5093)
- Chloé Bernhard
- Vincent Flacher (ORCID: https://orcid.org/0000-0002-8648-2615)
- Audrey A. Vincent
- Clémence Hubsch
- Samuel Meignan (ORCID: https://orcid.org/0000-0002-9386-7978)
- Chinar Salmanli
- Céline Frochot
- Sandra Raimbault
- Michel Salzet
- Georges Noel
- Mélodie Anne Karnoub
- Natacha Entz-Werlé
- Hélène Sudour-Bonnange
- Antonella Raffo-Romero
- Alessandro Furlan
- Sophie Pinel
- Andres Coca
- Cedric Boura
Institutions
- Centre National de la Recherche Scientifique (FR)
- Inserm (FR)
- Université de Lille (FR)
- Centre Hospitalier Universitaire de Lille (FR)
- Lille’s Cardiology Hospital (FR)
- Laboratoire de Biophotonique et Pharmacologie (FR)
- BC Platforms (Finland) (FI)
- Centre Hospitalier Régional et Universitaire de Nancy (FR)
- Levi Strauss (United States) (US)
- Délégation Centre-Est (FR)
- Centre de Recherche en Automatique de Nancy (FR)
- Centre de Recherche en Cancérologie de Lyon (FR)
- Centre Oscar Lambret (FR)
- Immunologie, Immunopathologie et Chimie Thérapeutique (FR)
- Institut de Biologie Moléculaire et Cellulaire (FR)
- Institut Polytechnique de Bordeaux (FR)
- Hôpital Civil, Strasbourg (FR)
- Université de Strasbourg (FR)
Publication Details
- Journal
- Cancer and Metastasis Reviews
- Published
- 2026-03-03
- DOI
- https://doi.org/10.1007/s10555-026-10316-3
- Primary Topic
- Glioma Diagnosis and Treatment
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Institut National Du Cancer