DNA-Encoded Libraries (DELs) for Discovering New Molecular Probes: Application to Live-Cell Bioimaging and Personalized Theranostics

While DNA-encoded libraries (DELs) are well recognized as valuable tools for the development of novel small-molecule therapeutics, their significant potential for developing new imaging probes initially received less attention. However, as DEL technology develops and novel screening modalities are introduced, several robust strategies for generating imaging probes from DEL screening campaigns have emerged. The current topical review aims to provide an overview of DEL technology as it relates to the discovery of new molecular probes and to present recent contributions that highlight innovative ways DELs are advancing the field. Approaches to harnessing DEL-derived probes for therapeutic applications, including their conversion into integrated theranostic modalities, will also be discussed.