Development of a point-of-care dual one-step recombinase-aided PCR assay for rapid identification of Mycobacterium tuberculosis gyrA mutations conferring fluoroquinolone resistance

The POCT-DO-RAP assay developed in this study achieves a fully integrated "sample-in, result-out" workflow on a single device, offering ultra-high sensitivity, precise mutation discrimination, and excellent clinical concordance. This approach provides a promising molecular diagnostic tool for rapid detection of drug-resistant tuberculosis, particularly suitable for primary healthcare and resource-limited settings.

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Publication Details

Journal
Frontiers in Microbiology
Published
2026-03-02
DOI
https://doi.org/10.3389/fmicb.2026.1772984
Primary Topic
Tuberculosis Research and Epidemiology
Type
article
Field-Weighted Citation Impact
0.00

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article

Development of a point-of-care dual one-step recombinase-aided PCR assay for rapid identification of Mycobacterium tuberculosis gyrA mutations conferring fluoroquinolone resistance

Qian Ma, Xi Liu, Zijin Zhao, Duoxiao Zhang et al.
Frontiers in Microbiology
Tuberculosis Research and Epidemiology
article

Development of a point-of-care dual one-step recombinase-aided PCR assay for rapid identification of Mycobacterium tuberculosis gyrA mutations conferring fluoroquinolone resistance

Qian Ma, Xi Liu, Zijin Zhao, Duoxiao Zhang, Yanqing Tie, Shijue Gao, Yujie Xiang, Xinxin Shen, Jianing Wu, Shihao Jiao, Junkai Ren, Zhiqiang Han, Xinxin Li, Xuejun Ma, Yuanrui Li, Kenan Peng
article en

Abstract

The POCT-DO-RAP assay developed in this study achieves a fully integrated "sample-in, result-out" workflow on a single device, offering ultra-high sensitivity, precise mutation discrimination, and excellent clinical concordance. This approach provides a promising molecular diagnostic tool for rapid detection of drug-resistant tuberculosis, particularly suitable for primary healthcare and resource-limited settings.

Frontiers in MicrobiologyVol. 17
Ningbo University (CN), Hebei Medical University (CN), North China University of Science and Technology (CN), Hebei North University (CN), HBIS (China) (CN), Hebei General Hospital (CN), National Institute for Viral Disease Control and Prevention (CN), Ningbo College of Health Sciences (CN)
National Natural Science Foundation of China, Chinese Center for Disease Control and Prevention
Peace, Justice and strong institutions
Openalex Percentile: Top 37%
Tuberculosis Research and Epidemiology
DOIOpen Access PDFOpenAlex
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Development of a point-of-care dual one-step recombinase-aided PCR assay for rapid identification of Mycobacterium tuberculosis gyrA mutations conferring fluoroquinolone resistance — Qian Ma, Xi Liu, et al. · Frontiers in Microbiology (2026) | TGRS Research Map | TGRS