Optimization of Recombinant Human Poly(ADP-ribose) Polymerase 3 Production

PARP3 (Poly(ADP-ribose) polymerase 3), like other PARP family members (PARP1 and PARP2), is an important factor in DNA repair. The specific functions and molecular mechanisms of action of this protein remain insufficiently studied. The development of a reliable protocol for obtaining high-purity and yield PARP3 is essential for comprehensive protein analysis, enzymatic activity, including studies of its binding to DNA, interactions with other protein factors, and structural investigations. In this study, we present a modified protocol for the expression of human PARP3 from Escherichia coli cells and its subsequent purification, which significantly increases protein yield compared to previously published methods.