An RNAi-based platform for spatiotemporal control of histone gene expression during animal development
We conclude that shRNA-mediated depletion of endogenous histone H3.2, coupled with histone replacement transgenes engineered to be insensitive to RNAi, is an effective experimental approach for studying the role of histone PTMs in animal development.
Authors
- Olga Arroyo
- Mary Leatham‐Jensen
- Daniel J. McKay
Institutions
- University of North Carolina at Chapel Hill (US)
Publication Details
- Journal
- Epigenetics & Chromatin
- Published
- 2026-03-02
- DOI
- https://doi.org/10.1186/s13072-025-00657-8
- Primary Topic
- Genomics and Chromatin Dynamics
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Institutes of Health
- National Institute of General Medical Sciences
- National Institute of Child Health and Human Development