A Fluorescent Aptasensor Based on Tb-MOFs and Its Application for the Detection of Pseudomonas aeruginosa in Foods

<i>Pseudomonas aeruginosa</i> is an important waterborne pathogen that is harmful to food safety and human health. Therefore, the detection of <i>Pseudomonas aeruginosa</i> has become an essential item in food sampling. This article constructs a fluorescent aptamer sensor based on Tb-MOFs, using Tb-MOFs as the fluorescence signal source, colloidal gold (AuNPs) as the signal conversion switch, and aptamers as the target recognition element, to establish a quantitative detection method for <i>Pseudomonas aeruginosa</i>. The constructed sensor exhibits a good linear relationship within the concentration range of <i>Pseudomonas aeruginosa</i> from 1 to 10⁶ CFU/mL, with a detection limit of 0.63 CFU/mL. Moreover, the sensor was also applied to the detection of <i>Pseudomonas aeruginosa</i> in actual samples of bottled drinking water and orange juice. The fluorescent aptasensor based on Tb-MOFs provides a rapid and sensitive new sensing method for the detection of <i>Pseudomonas aeruginosa</i> in food.