On the Development of a Genetically Encoded Photoconvertible Green Fluorescent Protein Based on PA-GFP

Abstract A photoconvertible fluorescent protein based on PAGFP, named PC-mGFP, is developed using molecular evolution in a bacterial system. PC-mGFP undergoes photoconversion from the green fluorescent state with a large Stokes shift to the green fluorescent state when irradiated with 405-nm light. The properties of PC-mGFP are characterized in vitro and in the cytosol of mammalian cells. PC-mGFP can be applied in the field of cellular imaging.