A preliminary investigation on the influence of sample storage on the low-abundant ocular surface microbiome
In this study, the impact of sample freezing prior to DNA extraction was investigated on a small sample set (n = 6) to determine whether this affects the composition of the ocular surface microbiome - a low biomass environment. Frozen samples exhibited a reduced detection of Gram-negative bacteria compared to directly processed samples.
Authors
- Elio L. Herzog (ORCID: https://orcid.org/0000-0001-9403-919X)
- Martin Sebastian Zinkernagel (ORCID: https://orcid.org/0000-0003-3447-2359)
- Marco Kreuzer (ORCID: https://orcid.org/0000-0001-6784-0854)
- Denise C. Zysset‐Burri (ORCID: https://orcid.org/0000-0002-4147-5374)
- Livia A. Spörri
- Jérémy Rotzetter
Institutions
- University of Bern (CH)
- SIB Swiss Institute of Bioinformatics (CH)
- University Hospital of Bern (CH)
Publication Details
- Journal
- Journal of Microbiological Methods
- Published
- 2026-03-03
- DOI
- https://doi.org/10.1016/j.mimet.2026.107437
- Primary Topic
- Ocular Infections and Treatments
- Type
- article
- Field-Weighted Citation Impact
- 0.00