Inter-laboratory Evaluation of γH2AX/53BP1 DNA Double-strand Break Foci Assays in Human Lymphocytes Under Low-dose Irradiation: Implications for Calyculin A

These findings demonstrate that inter-laboratory reproducibility of γH2AX/53BP1-based biodosimetry is achieved primarily through disciplined workflow harmonization rather than through pharmacological enhancement. By reinforcing assay reproducibility and biological consistency, this work supports the translational applicability of IRIF-based biodosimetry for broader application in radiation exposure assessment.