INVESTIGATION OF THE NEUROPROTECTIVE EFFECT OF METFORMIN AGAINST PENICILLIN-INDUCED NEUROTOXICITY IN SH-SY5Y CELL LINE

Introduction: Neurodegenerative diseases such as Alzheimer’s and Parkinson’s cause necrosis of neurons, loss of function, abnormal signal transmissionbetween cells, inflammatory response and insulin dysfunction in the brain. Itis estimated that this situation will affect approximately 150 million peopleglobally in 2050 and cause an economic burden of 10 trillion dollars (Temple, 2023). It is thought that metformin, a guanidine-derived antidiabeticagent, may play a neuroprotective role by regulating energy metabolism, oxidative stress, inflammatory response, and preventing neuronal dysfunctionand neuron death (Sharma et al., 2021; Du et al., 2022). This study aimed toinvestigate the neuroprotective effect of metformin against penicillin-inducedneurotoxicity in the SH-SY5Y cell line. Materials and Methods: After seeding SH-SY5Y cells, various concentrations of penicillin and metformin were applied, and at the end of 24 hours,neuronal viability test (MTT) was performed to decide the concentrations of penicillin and metformin for the study. The effect of metformin (10, 20, 40mM) against penicillin (800 µM)-induced neurotoxicity in SH-SY5Y cellswas tested with three different experimental designs (pre-treatment, co-treatment, post-treatment). Results: Metformin (10, 20, 40 mM) showed a neuroprotective effect inpre-treatment and co-treatment experimental designs, while metformin (40mM) showed a neuroprotective effect post treatment. Conclusions: After this stage, we will continue to confirm the neuroprotective effect of metformin in our studies and focus on elucidating the underlying mechanisms. <br>