Determination of extraction conditions for triterpenic compounds of japanese quince (Chaenomeles japonica (Thunb.) Lindl. ex Spach) fruit samples

Introduction. The selection of an extractant and extraction conditions is crucial for developing methods to determine plant bioactive compounds. For extracting triterpenic compounds, non-polar solvents like acetone, alcohols (ethanol, methanol), chloroform, and their mixtures, are commonly utilized [1]. Materials and methods. 1 g of lyophilized Japanese quince was placed in dark glass bottles, covered with 10 ml of acetone, and placed in an ultrasonic bath (80 kHz). The extracts were filtered through a vacuum pump and 0.2 μm membrane filters before HPLC analysis [1]. Results were analyzed using MS Excel (Microsoft, Redmond, WA, USA). Results and their discussion. Firstly, the effect of an extractant on the recovery of triterpenic compounds from Japanese quince was evaluated. The extraction solvents chosen were methanol 100% (v/v), ethanol 96% (v/v), and acetone 100% (v/v). The highest yield (4388.82 ± 259.11 µg/g) was obtained with acetone. Secondly, the effect of extraction time, ranging from 10 minutes to 60 minutes was examined. The highest yield (4174.72 ± 94.97 µg/g) was obtained in 50 minutes. Finally, the effect of ultrasonic power was investigated. The best yield (4616.92 ± 72.31 µg/g) was obtained using a 565 W ultrasonic power. In conclusion of this research, the highest yield of triterpenic compounds from Japanese quince fruits was obtained extracting samples with a 100% acetone for 50 minutes using 565 W ultrasonic power. These conditions were selected for further research of Japanese quince fruits.