Characterization and Molecular Cloning of CRABP Gene in Brown Trout (Salmo trutta)

The aim of this study is to obtain the cDNA of the CRABP (cellular retinoic acid binding protein) of brown trout (Salmo trutta) by using Bioinformatics methods and molecular cloning methods. Our second aim is to determine the phylogenetic connections of the crabp gene with other living species, to extract its nucleotide sequences, to reach its genomic structure and to obtain gene transcripts. Finally, we obtained mRNA expressions and tissue-specific distributions of the stress factors that we will apply to the liver tissues of brown trout.