Evaluation of the Effects of Alpha‐Amylase and Beta-Glucanase (Optipartum C+ 200) on the Health and Metabolic Profile of Lactating Dairy Cows

Objectives: This study seeks to examine the effects of feed supplements containing alpha- -amylase and beta­ glucanase (Optipartum C+ 200) on the health and metabolic profile of lactating dairy cows, both during the lactation period and under conditions of heat stress. Methods: This study was conducted with two groups of lactating dairy cows. The control group (n=20) received only a standard total mixed ration (TMR), while the treatment group (n=20) was supplemented with 200 g/day/cow of Optipartum­ C+. Heat stress was assessed using the Temperature­ Humidity Index (THI) at four distinct time points: August 14, 2024 (THI = 76.96), September 11, 2024 (THI = 70.4), October 9, 2024 (THI = 63.24), and November 6, 2024 (THI = 54.86). Blood samples were collected at each time point for biochemical analysis, and cows were categorized according to their lactation stage. The following blood parameters were measured: urea (UREA), glucose (GLU), aspartate aminotransferase (AST), potassium (K), calcium (Ca), magnesium (Mg), phosphorus (P), gamma­ glutamyltransferase (GGT), and beta- -hydroxybutyrate (BHB). The data from both groups were compared for these parameters over the duration of the study. Results: Throughout the study, blood urea and glucose levels remained within normal ranges, with the treatment group showing lower urea and glucose levels than the control group in August (THI – 76,96), September (THI – 70,4), and October (THI – 63,24). Liver enzyme activity (AST) was lower in the treatment group, indicating positive effects on liver function under heat stress. GGT levels were elevated in both groups, but the treatment group showed a decrease, suggesting improved metabolic health. The treatment group had lower BHB levels, indicating better energy metabolism and reduced ketone production, particularly during heat stress. Additionally, the treatment group showed higher milk yield and a more favorable milk fat­ to­ protein ratio, reflecting better rumen function and energy metabolism. Milk protein levels in the treatment group were closer to the normal range and remained stable, while the control group had lower protein levels. Milk lactose levels in the treatment group stayed within normal limits, while the control group’s lactose levels decreased, likely due to reduced glucose availability. No differences were observed in blood potassium, calcium, magnesium, or phosphorus levels, suggesting no effect of the feed supplement on these parameters. Conclusions: Cows in the treatment group showed improved energy metabolism, with lower AST and GGT activity, indicating reduced liver strain and metabolic stress. Lower blood urea levels suggested better protein digestion and optimized rumen fermentation. Blood glucose was slightly lower but efficient, supporting milk lactose synthesis and reducing metabolic stress. BHB levels were lower, indicating reduced fat mobilization and ketosis risk. The treatment group had more stable metabolic parameters during and after heat stress, including lower AST, GGT, and BHB levels, highlighting improved metabolism and reduced liver damage. Milk production, fat, protein, and lactose levels were stabilized, and body fat mobilization was reduced. Overall, Optipartum­ C+ (200 g/day) effectively mitigated heat stress and improved cow health and productivity.