The effect of bioelectronic technology application on boar sperm viability, motility, and pH stability

Innovative bioelectronic technologies that can activate and influence surrounding cells and their processes hold significant promise for improving sperm functionality and preservation. The objective of this study was to evaluate the impact of the Bioelectronic technology prototype on sperm viability, motility, and pH stability incubating diluted boar semen at a temperature of 17 °C for 6 hours. The study used 13 doses of diluted boar semen obtained from a commercial pig farm. Each dose of semen was poured into three equal 30 ml storage tubes. The samples in the tubes were divided into three experimental groups: Control, Bio1short (sample kept on 1 biosensor plate) and Bio4short (sample kept on a 4-biosensor package). All samples were kept in a thermostat maintained at +17 °C. Sperm motility, viability and pH were tested according to the methods used in the lab at two time points: 0 hour (Control sample) and 6 hours (Control, Bio1short and Bio4short). After 6 hours, both experimental groups demonstrated significantly higher motility than the control (p < 0.05). The control group showed a statistically significant decrease in sperm motility from 93.90% (SD = 0.85) to 90.95% (SD = 1.34) (p < 0.05), whereas motility remained stable in the Bio1short (93.19%, SD = 0.89) and Bio4short (93.70%, SD = 0.89) groups. Sperm viability declined in the control from 94.62% (SD = 0.75) to 92.23% (SD = 1.65) (p < 0.05), but remained higher in Bio1short (94.15%) (SD = 1.01) and Bio4short (93.69%) (SD = 1.13) (p < 0.05). The results obtained indicate that the use of Bioelectronic technology prototype can have a positive effect on boar semen quality indicators by helping to maintain higher sperm viability, motility, and medium stability during storage.